Proglucagon ELISA

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The Proglucagon Enzyme Linked Immunosorbent Assay (ELISA) kit provides materials for the quantitative measurement of Proglucagon in human plasma and other biological fluids. No detectable cross-reactivity to Glucagon, GLP-1, GLP-2, Oxyntomodulin, GRPP, MPGF-1, MPGF-2, Insulin, C-Peptide, or Thyroglobulin.

 

 

Catalog Number

AL-1019

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Packaging

96 well microtiter

Detection

HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter

Dynamic Range

6, 6.48 – 913 pg/mL

Limit of Detection

1.14 pg/mL

Sample Size

25 µL

Sample Type

Plasma

Assay Time

2.5 hours

Species Reactivity

Human

Shelf Life

24 months

Availability

Worldwide

Proglucagon, a 158 amino acid polypeptide, is synthesized in the pancreatic alpha cells and the intestinal L-cells1. Its post-translational processing in the pancreas leads to GRPP, Glucagon, IP-1 and MPGF. While in the intestines, the post-translational process leads to Glicentin, Oxyntomodulin, GLP-1, IP-2 and GLP-2.2 Analogs and agonists of these proglucagon factors are used in the treatment and management of Type-1 and Type-2 diabetes and prevention of cardiovascular complications associated with diabetes.

References:

  1. Jacques Philippe, Svetlana Mojsov, Daniel J. Drucker, Joel F. Habener., Proglucagon Processing in a Rat Islet Cell Line Resembles Phenotype of Intestine Rather than Pancreas, Endocrinology, Volume 119, Issue 6, 1 December 1986, Pages 2833–2839.
  2. Raffort, J., Lareyre, F., Massalou, D., Fénichel, P., Panaïa-Ferrari, P., & Chinetti, G. (2017). Insights on glicentin, a promising peptide of the proglucagon family. Biochemia medica, 27(2), 308–324.

Technical Literature