Glucagon ELISA

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The Glucagon ELISA kit measures Glucagon (1-29) with no detectable cross-reactivity to Glicentin, Oxyntomodulin, GLP-1, GLP-2, or GRPP. Simple procedure, 2.5 hour incubations, no extraction needed, no special collection tube required. Relevant dynamic range and sensitivity.



Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.


96 well microtiter


HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter

Dynamic Range

6, 7-314 pg/mL

Limit of Detection

2.4 pg/mL

Sample Size

50 µL

Sample Type


Assay Time

2.5 hours

Species Reactivity

Human, Mouse

Shelf Life

24 months



Glucagon and Oxyntomodulin, a peptide hormone secreted by the alpha cells of pancreas, share identical amino acid sequence in the N-terminal 29 aa. Glucagon is a 29-amino acid polypeptide processed from proglucagon in pancreatic alpha cells.1 In intestinal L-cells proglucagon is cleaved into glicentin, corresponding to proglucagon residues no 1-69. Glicentin can further be processed into oxyntomodulin, corresponding to proglucagon residues no 33-69. These peptides are released simultaneously upon stimulation.

Glucagon has shown to have an effect opposite to that of insulin, i.e. it raises blood glucose levels. It causes the liver to convert glycogen into glucose, which is then released into the blood stream.2-4 During hypoglycaemia, glucagon secretion offers a protective feedback mechanism, defending the organism against damaging effects of glucose deficiency in the brain and nerves.5


  1. Sandoval DA and D’Alessio DA. Physiology of Proglucagon Peptides: Role of Glucagon and GLP-1 in Health and Disease. Physiol Rev 95:513-548.
  2. Bagger JI et al (2011) Glucagon antagonism as a potential therapeutic target in type 2 diabetes. Diabetes, Obesity and Metabolism 13:965-971.
  3. Holst J et al (2004) Role of incretin hormones in the regulation of insulin secretion in diabetic and nondiabetic humans. Am J Physiol Endocrinol Metab 287: E199-206.
  4. Holst J (2010) Glucagon and Glucagon-Like Peptides 1 and 2. Results Probl Cell Differ 50:121-135.
  5. Young A (2005) Inhibition of Glucagon Secretion. Advances in Pharmacology 52:151-171.

Glucagon ELISA AL‐157


Kumar A, Kalra B, Mistry S, Patel AS, Budhavarapu V, Chatterji T, Savjani G, Krishna R, D’Alessio D. Development of Novel Specific and Sensitive ELISAs for Proglucagon‐Derived Peptides. Poster presented at 69th AACC Meeting; 2017 July 30‐Aug 3; San Diego, CA

All Products Cited: Glucagon ELISA AL‐157, GLP‐1 ELISA AL‐172, Oxyntomodulin ELISA AL‐139

Technical Literature