The Canine Anti-Müllerian hormone (AMH) enzyme-linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of AMH in Canine serum. The test is intended to be used as a diagnostic tool in the determination of functional gonadal (spayed, neutered or Intact) status in dogs.
| Catalog Number | AL-116 |
|---|---|
| Regulatory Status | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 2, 0.3-11.9 ng/mL |
| Limit of Detection | 0.15 ng/mL |
| Sample Size | 25 µL |
| Sample Type | Serum |
| Assay Time | 3.5 hours |
| Species Reactivity | Canine, Feline |
| Shelf Life | 24 months |
| Availability | Worldwide |
Anti-Müllerian hormone is a 140 kDa glycoprotein that is produced during normal embryogenesis by the Sertoli cells of the embryonic testis, causes the involution of the Müllerian duct, and inhibits female gonadogenesis by inducing apoptosis of target gonadal cells. It belongs to the transforming growth factor-β super family. AMH causes apoptosis of specific Anti-Müllerian inhibiting substance (MIS) receptor-bearing cells, while having no effect on cells without receptors. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary, and AMH inhibits recruitment of primordial follicles into the pool of growing follicles, and decreases responsiveness of growing follicles to FSH.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
AMH (Canine) ELISA AL-116
Cusatti AC, Somoza R, Marchetti IA. Antimullerian Hormone (AMH) as biomarked of Granulosa Cell Tumor (GCT), case report. Abstract presented at the 20th European Veterinary Society for Small Animal Reproduction Congress, 29th June – 1st July 2017, Vienna Austria
All Products Cited Canine AMH ELISA, AL-116
Hollinshead FK, Walker C, Hanlon DW. Determination of the normal reference interval for anti-Müllerian hormone (AMH) in bitches and use of AMH as a potential predictor of litter size. Reprod Domest Anim. 2017 Apr;52 Suppl 2:35-40. PMID 27757997
All Products Cited Canine AMH ELISA AL-116
Kumar A, Kalra B, Patel A, Shah S. New Sensitive Anti-Müllerian Hormone (AMH) ELISAs for Non-Human Primate, Rodent, Equine, Bovine, Canine and Other Species. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
All Products Cited Human/Primate AMH ELISA AL-105; Bovine AMH ELISA AL-114; Canine AMH ELISA AL-116; Equine and Ovine AMH ELISA AL-115; Rat and Mouse AMH ELISA AL-113
Themmen APN, Kalra B, Visser JA, Kumar A, Savjani G, Gier J, Jaques S. The use of anti-Müllerian hormone as diagnostic for gonadectomy status in dogs. Theriogenology 86 (2016) 1467–1474. PMID 27291082
All Products Cited AMH (Canine) ELISA AL-116; rec Hu AMH Ag AG-303-BA047
Yilmaz O, Toydemir T, Kirsan I, Ucmak Z, Karacam E. Anti-Mullerian hormone as a diagnostic tool for ovarian remnant syndrome in bitches. Vet Res Commun. First published online June 24, 2015. PMID 26099303
All Products Cited Canine AMH ELISA AL-116