The Equine Anti-Müllerian hormone (AMH) enzyme-linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of AMH in equine serum and other biological fluids.
| Regulatory Status | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
|---|---|
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 2, 0.06-14 ng/mL |
| Limit of Detection | 0.009 ng/mL |
| Sample Size | 50 µL |
| Sample Type | Serum |
| Assay Time | 3.5 hours |
| Species Reactivity | Equine |
| Shelf Life | 24 months |
| Availability | Worldwide |
Anti-Müllerian hormone is a 140 kDa glycoprotein that is produced during normal embryogenesis by the Sertoli cells of the embryonic testis, causes the involution of the Müllerian duct, and inhibits female gonadogenesis by inducing apoptosis of target gonadal cells. It belongs to the transforming growth factor-β super family. AMH causes apoptosis of specific Anti-Müllerian inhibiting substance (MIS) receptor-bearing cells, while having no effect on cells without receptors. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary, and AMH inhibits recruitment of primordial follicles into the pool of growing follicles, and decreases responsiveness of growing follicles to FSH.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
AMH (Equine) ELISA AL-115
Acharya M, Burke JM, Anshworth AJ, Rorie R Relationship of Anti-Mullerian Hormone to Reproductive Traits in Katahdin Ewes Bred in Late Spring or Fall Advances in Reproductive Sciences January 2020 08(01):48-56. DOI: 10.4236/arsci.2020.81005
All Products Cited: Equine and Ovine AMH ELISA AL-115
Kumar A, Kalra B, Patel A, Shah S. New Sensitive Anti-Müllerian Hormone (AMH) ELISAs for Non-Human Primate, Rodent, Equine, Bovine, Canine and Other Species. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
All Products Cited Human/Primate AMH ELISA AL-105; Bovine AMH ELISA AL-114; Canine AMH ELISA AL-116; Equine and Ovine AMH ELISA AL-115; Rat and Mouse AMH ELISA AL-113
Scarlet D, Kuhl J, Wulf M, Ille N, Aurich C. Antral Follicle Count of 2-Year-Old Mares is Highly Correlated with Anti-Müllerian Hormone Concentrations at 24 to 28 Weeks of Age. Reprod Fertil Dev. 2016 Jan;29(1):168-169.
All Products Cited Equine AMH ELISA AL-115
Scarlet D, Wulf M, Kuhl J, Köhne M, Ille N, Conley AJ, Aurich C. Anti-Müllerian hormone profiling in prepubertal horses and its relationship with gonadal function. Theriogenology. 2018 Sep 1;117:72-77. doi:10.1016/j.theriogenology.2018.05.012. Epub 2018 May 15. PubMed PMID: 29784463.
All Products Cited: Equine AMH ELISA AL-115