The Bovine AMH ELISA is a quantitative three-step sandwich type immunoassay.  In the first step serially diluted Calibrators and unknown samples are added to AMH antibody coated micro titer wells and incubated. After the first incubation and washing, the wells are incubated with biotinylated AMH antibody solution. After second incubation and washing, the wells are incubated with streptavidin horseradish peroxidase conjugate (SHRP) solution.  After the third incubation and washing step, the wells are incubated with substrate solution (TMB) followed by an acidic stopping solution. In principle, the antibody-biotin conjugate binds to the solid phase antibody-antigen complex which in turn binds to the streptavidin-enzyme conjugate. The antibody-antigen-biotin conjugate-SHRP complex bound to the well is detected by enzyme-substrate reaction. The degree of enzymatic turnover of the substrate is determined by dual wavelength absorbance measurement at 450 nm as primary test filter and 630 nm as reference filter. The absorbance measured is directly proportional to the concentration of AMH in the samples and calibrators.

AMH (Bovine) CLIA AL-214 and ELISA AL-114

Berdugo J, Tarazona-Moraels A, Echeverry-Zuluaga J, Cordona Maya W, Lopez-Herrera A, Crudeli G, Konrad J, Espasandin G. Antimullerian hormone levels in different bovine species and the relationship with in vitro embryo production Poster Presentation at the International Embryo Transfer Society, Jan 16-19, 2020, New York, New York.

All Products Cited: AMH (Bovine) ELISA AL-114

Center K, Dixon D, Looney C, Rorie R Anti-Mullerian Hormone and Follicle Counts as Predictors of Superovulatory Response and Embryo Production in Beef Cattle Advances in Reproductive Sciences. January 2018 06(01):22-33. DOI: 10.4236/arsci.2018.61003

All Products Cited: AMH (Bovine) ELISA AL-114

Gobikrushanth M, Dutra PA, Bruinje TC, Colazo MG, Butler ST, Ambrose DJ. Repeatability of antral follicle counts and anti-Müllerian hormone and their associations determined at an unknown stage of follicular growth and an expected day of follicular wave emergence in dairy cows. Theriogenology. 2017 Apr 1;92:90-94. Epub 2017 Jan 11. PMID 28237349

All Products Cited Bovine AMH CLIA AL-214

Gobikrushanth M, Dutra PA, Felton CA, Ruiz-Sanchez A, Bruinje TC, Colazo MG, Butler ST, Ambrose DJ. The association between Anti-Mullerian Hormone concentrations, antral follicle count and fertility measures in dairy cows. Presented at ADSA-ASAS-CSAS-WSASAS Joint Annual Meeting July 19-23, Salt Lake City, UT, Poster # F 17877.

All Products Cited AMH (Bovine) CLIA AL-214

Gobikrushanth M, Dutra PA, Felton CA, Ruiz-Sanchez A, Bruinje TC, Colazo MG, Butler ST, Ambrose DJ. The repeatability of antral follicle count and anti-Müllerian hormone concentration at two different postpartum stages in dairy cattle. Presented at ADSA-ASAS-CSAS-WSASAS Joint Annual Meeting July 19-23, Salt Lake City, UT, Poster # W 17841.

All Products Cited AMH (Bovine) CLIA AL-214

Jaques JT, Cardoso RC, Chachere J, Sinha S Using Anti-Müllerian Hormone (AMH) as a Predictor of Ova Production for Bovine Embryo Transfer Advances in Reproductive Sciences January 2020 08(01):36-47. DOI: 10.4236/arsci.2020.81004

All Products Cited: AMH (Bovine) ELISA AL-114

Konrad J, Berdugo Gutierrez JA, Yuponi R, Vallejos N, Cardona-Maya W, Clerico G, Crudell G, Sansinena M. Superstimulation effect on in vitro embryo production and relationship with anti-mullerian hormone in buffaloes (Bubalus bubalis) aspirated 7 or 14 days after follicular ablation. Poster Presentation at the International Embryo Transfer Society, Jan 16-19, 2020, New York, New York.

All Products Cited: AMH (Bovine) ELISA AL-114

Ribeiro ES, Bisinotto RS, Lima FS, Greco LF, Morrison A, Kumar A, Thatcher WW, Santos JE. Plasma anti-Müllerian hormone in adult dairy cows and associations with fertility. Journal of Dairy Science. 2014 Nov;97(11):6888-900. PMID 25173464

All Products Cited AMH (Bovine) CLIA AL-214

Ribeiro ES, Cerri R, Bisinotto R, Lima F, Greco L, Morrison A, Kumar A, Thatcher W, Santos J. Associations Between Plasma Anti-Müllerian Hormone and Fertility Responses of Seasonally Calving Grazing Dairy Cows. Poster session presented at American Dairy Science Association; 2012; July 15-19; Phoenix, AZ.

All Products Cited AMH (Bovine) CLIA AL-214, AMH (Bovine) ELISA AL-114

Ribeiro ES, Greco LF, Bisinotto RS, Lima FS, Thatcher WW, Santos JE. Biology of Preimplantation Conceptus at the Onset of Elongation in Dairy Cows. Biol Reprod. 2016 Apr;94(4):97. doi: 10.1095/biolreprod.115.134908. Epub 2016 Mar 2. PMID: 26935601.

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Xu J, Xu F, Lawson MS, Tkachenko OY, Ting AY, Kahl CA, Park BS, Stouffer RR, Bishop CV. Anti‐Müllerian hormone is a survival factor and promotes the growth of rhesus macaque preantral follicles during matrixfree culture. Biol Reprod. 2017 Dec 27.

All Products Cited: AMH (Bovine) CLIA AL‐205

AMH (Bovine) ELISA AL-114

Akbarinejad V, Gharagozlou F, Vojgani M. Temporal effect of maternal heat stress during gestation on the fertility and anti-Müllerian hormone concentration of offspring in bovine. Theriogenology. 2017 Sep 1;99:69-78. PMID 28708502

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Baldrighi JM, Sá Filho MF, Batista EO, Lopes RN, Visintin JA, Baruselli PS, Assumpção ME. Anti-Mullerian hormone concentration and antral ovarian follicle population in Murrah heifers compared to Holstein and Gyr kept under the same management. Reproduction in Domestic Animals. 2014 Dec; 49(6):1015-20. PMID 25291989

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Baruselli PS, Batista EOS, Vieira LM, Souza Ah. Relationship between follicle population, AMH concentration and fertility in cattle. Anim Reprod. 2015 Sep. 12 (3): 487-497

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Batistia E, Guerreiro BM, Freitas BG, Silva JCB, Vieira LM, Ferreira RM, Rezende RG, Basso AC, Lopes, R, Renno FP, Souza AH, Baruselli PS. Plasma anti-Mullerian hormone as predictive endocrine marker to select Bos taurus (Holstein) and Bos indicus (Nelore) calves for in vitro embryo production. Domestic Animal Endocrinology, 2015 Aug 54: 1-9 epub ahead of print. PMID 26379099

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Batistia E, Macedo G, Sala R, Ortolan M, Sa Filho M, Del Valle T, Jesus E, Lopes R, Renno F, Baruselli P. Plasma Anti-mullerian Hormone as a Predictor of Ovarian Antral Follicular Population in Bos indicus (Nelore) and Bos Taurus (Holstein) Heifers. Reproduction in Domestic Animals. 2014 Jun; 49(3):448-452. PMID 24689827

All Products Cited AMH (Bovine) ELISA AL-114

Berdugo J, Tarazona-Moraels A, Echeverry-Zuluaga J, Cordona Maya W, Lopez-Herrera A, Crudeli G, Konrad J, Espasandin G. Antimullerian hormone levels in different bovine species and the relationship with in vitro embryo production Poster Presentation at the International Embryo Transfer Society, Jan 16-19, 2020, New York, New York.

All Products Cited: AMH (Bovine) ELISA AL-114

Bo G, Anduaga I, Bernal B, Moreira G, Cedeno A, Barajas J, Pelizzari M, Andrada S, Tribulo A. Serum AMH concentrations and in vitro embryo production in beef cattle. Poster session presented at 42nd International Embryo Transfer Society Annual Meeting; 2016 Jan; Louisville, Kentucky

All Products Cited AMH (Bovine) ELISA AL-114

Cardoso CJT, Oliveira Junior JS, Kischel H, Silva WAL, Arruda EDS, Souza-Cáceres MB, Oliveira F A M, Nogueira E, Nogueira GP, Melo-Sterza FA Anti-Müllerian hormone (AMH) as a predictor of antral follicle population in heifers. Animal Reproduction, v. 15, p. 12-16, 2018. (598)

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Carter, AS. Anti-Mullerian Hormone as a Marker of Oocyte Quantity, Developmental Potential, and Fetal Sex. [Dissertation, 2016]. Ontario (Canada): The University of Guelph.

All Products Cited Bovine AMH ELISA AL-114

Center K, Dixon D, Looney C, Rorie R Anti-Mullerian Hormone and Follicle Counts as Predictors of Superovulatory Response and Embryo Production in Beef Cattle Advances in Reproductive Sciences. January 2018 06(01):22-33. DOI: 10.4236/arsci.2018.61003

All Products Cited: AMH (Bovine) ELISA AL-114

Chachere JA. Anti-Mullerian Hormone Concentrations as a Predictor of Superovulation Response and Conception Rate in Beef Females. [Thesis].College Station (TX)]: Texas A&M University.

All Products Cited AMH (Bovine) ELISA AL-114

Copping KJ, Ruiz-Diaz MD, Rutland CS, Mongan NP, Callaghan MJ, McMillen IC, Rodgers RJ, Perry VEA. Periconception and first trimester diet modifies reproductive development in bulls. Reprod Fertil Dev. 2017 Nov 16.

All Products Cited: AMH (Bovine) ELISA AL-114

Gobikrushanth M, Dutra PA, Bruinjé TC, Colazo MG, Butler ST, Ambrose DJ. Repeatability of antral follicle counts and anti-Müllerian hormone and their associations determined at an unknown stage of follicular growth and an expected day of follicular wave emergence in dairy cows. Theriogenology. 2017 Apr 1;92:90-94 PMID 28237349

All Products Cited Bovine AMH ELISA AL-114

Guerreiro BM, Batista EO, Vieira LM, Sá Filho MF, Rodrigues CA, Castro Netto A, Silveira CR, Bayeux BM, Dias EA, Monteiro FM, Accorsi M, Lopes RN, Baruselli PS. Plasma anti-mullerian hormone: an endocrine marker for in vitro embryo production from Bos taurus and Bos indicus donors. Domestic Animal Endocrinology. 2014 Oct; 49:96-104. PMID 25136816

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Ilha GF, Rovani MT, Gasperin BG, Ferreira R, de Macedo MP, Neto OA, Duggavathi R, Bordignon V, Gonçalves P. Regulation of Anti-Mullerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle. Reprod Domest Anim. 2016 Apr;51(2):188-94. Epub 2016 Jan 27. PMID 26815645

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Iván AM, Cedeño A, Tríbulo A, Tapia C, Mata J, Mata C, Cavallero J, Bo G. HORMONA ANTIMULLERIANA (AMH) Y PRODUCIÓN INVITRO DE EMBRIONES. June 2017 Argentina [unknown where it was ultimately published].

All Products Cited Bovine AMH ELISA AL-114

Jaques JT, Cardoso RC, Chachere J, Sinha S Using Anti-Müllerian Hormone (AMH) as a Predictor of Ova Production for Bovine Embryo Transfer Advances in Reproductive Sciences January 2020 08(01):36-47. DOI: 10.4236/arsci.2020.81004

All Products Cited: AMH (Bovine) ELISA AL-114

Konrad J, Berdugo Gutierrez JA, Yuponi R, Vallejos N, Cardona-Maya W, Clerico G, Crudell G, Sansinena M. Superstimulation effect on in vitro embryo production and relationship with anti-mullerian hormone in buffaloes (Bubalus bubalis) aspirated 7 or 14 days after follicular ablation. Poster Presentation at the International Embryo Transfer Society, Jan 16-19, 2020, New York, New York.

All Products Cited: AMH (Bovine) ELISA AL-114

Kumar A, Kalra B, Patel A, Shah S. New Sensitive Anti-Müllerian Hormone (AMH) ELISAs for Non-Human Primate, Rodent, Equine, Bovine, Canine and Other Species. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.

All Products Cited Human/Primate AMH ELISA AL-105; Bovine AMH ELISA AL-114; Canine AMH ELISA AL-116; Equine and Ovine AMH ELISA AL-115; Rat and Mouse AMH ELISA AL-113

Lopes Dias Queiroz V. Anti-Mullerian Serum Hormone as a Predictor of Puberty in Young Nelore Bulls [Dissertation, 2014]. Mato Grosso (Brazil)]: Universidade Federal De Mato Grosso Do Sul.

All Products Cited Bovine AMH ELISA AL-114

Maculan R, Pinto TLC, Moreira GM, Vasconcelos GL, Sanches JA, Rosa RG, Bonfim RR, Gonçalves TM, Souza JC. Anti‐Müllerian Hormone (AMH), antral follicle count (AFC), external morphometrics and fertility in Tabapuã cows. Anim Reprod Sci. 2018 Feb;189:84‐92.

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Rorie RW, Newberry HR, Lester TD, Acharya M, Hansen CR. Anti-Müllerian Hormone at Weaning and Breeding as a Predictor of Beef Heifer Fertility. Reprod Fertil Dev. 2016 Jan;29(1):111-112.

All Products Cited Bovine AMH ELISA AL-114

Sakaguchi K, Yanagawa Y, Yoshioka K, Suda T, Kawano K, Katagiri S, Nagano M. Effects of antral follicle count in ovaries on follicular development and endocrine dynamics of follicle stimulating hormone and steroid hormones in cattle. Reproduction, Fertility and Development 31(1) 185-186, 2018. (609)

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Sequeira M, Guillen S, Meikle A, Viñoles C. Relación entre la máxima cuenta folicular, las concentraciones de hormona antimüleriana y la concepción en vaquillonas Hereford. Poster session presented at Proceedings del 2do Congreso de la Sociedad Argentina de Tecnologías Embrionari a Arbitrado; 2014; May26-27; Buenos Aires, Argentina.

All Products Cited Bovine AMH ELISA AL-114

Stojsin A, Sovernigo T, Filho M, Gillis D, Adona P, Miranda M, Ohashi O, Neal M, Madan P, King A. Plasma antimüllerian hormone predicts bovine ovary size, number of antral follicles, and overall animal fertility in three breeds of cattle. Poster session presented at Ovarian Club III; 2013 Nov; Paris France.

All Products Cited Bovine AMH ELISA AL-114

Vales C, Recavarren M, Ranochia R, Bianchi C, De La Mata A, De La Mata J, Alberio RH. Determinación de la hormona antimulleriana (AMH) en plasma de vacas superovuladas como predictora de la respuesta. Poster session presented at 2° Congreso de Sociedad Argentina de Tecnologías Embrionarias; 2014; May 28-31; Buenos Aires, AR.

All Products Cited Bovine AMH ELISA AL-114

Technical Documents