The Oocyte Maturation Quality (OMQ) Check enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of dimeric Inhibin A in human serum.
| Catalog Number | |
|---|---|
| Regulatory Status | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Packaging | 96 well microtiter |
| Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
| Dynamic Range | 6, 41-6027 pg/mL |
| Limit of Detection | 6.58 pg/mL |
| Sample Size | 50 µL |
| Sample Type | Serum |
| Assay Time | 1 hour |
| Species Reactivity | Bovine, Equine, Human |
| Shelf Life | 24 months |
| Availability | Worldwide |
Inhibins are heterodimeric protein hormones secreted by granulosa cells of the ovary in the female and Sertoli cells of the testis in the male. They selectively suppress the secretion of pituitary follicle stimulating hormone (FSH) and have local paracrine actions in the gonads. The fully processed form of the inhibin molecule has a molecular weight of approximately 32 kD and consists of the two distinct chains (α and β), linked by disulfide bridges. Higher molecular weight forms, with precursor forms of the α-subunit, also occur in follicular fluid and serum. In addition, free α-subunit forms, unassociated with a β-subunit, and lacking inhibin bioactivity, are also present. Inhibin A consists of an α-subunit and βA-subunit.
Recently, Inhibin-A and Inhibin-B are emerging as markers for the assessment of developing follicles during ovarian stimulation.1 and can assist with the follicle dynamics. The number of mature oocytes is a key factor in the IVF success. Gonadotropin dosing during ovarian stimulation cycle is a critical step in maximizing the oocytes yield. The serum estradiol and transvaginal ultrasound measurements are currently used as monitoring tools in stimulated cycle. Serum Estradiol levels are commonly supraphysiological and often variable due to multi-follicular growth of varying size of follicles in IVF cycles. Additionally, Estradiol binds to peripheral SHBG and the competitive nature of the immunoassay makes Estradiol measurement unreliable for checking the oocyte maturation quality. In contrast, serum Inhibin A levels increase only when follicle size are between 12–15 mm. This makes serum Inhibin-A a better marker for studying the Oocyte quality. Along with routine ultrasound measurements, Inhibin A has been studied for optimal timing of trigger medication with a threshold of ≥10 mature oocytes.1 Serum Inhibin-A levels strongly correlate to the number of follicles ≥15mm and mature oocytes. Measurements of Inhibin-A are shown to be useful in studying its role in human reproductive physiology.1
Reference:
1 Barbara Lawrenz, Leyla Depret Bixio, Carol Coughlan, Claus Yding Andersen, Laura Melado, Bhanu Kalra, Gopal Savjani, Human M. Fatemi and Ajay Kumar; Inhibin A- A promising predictive parameter for determination of final oocyte maturation in ovarian stimulation for IVF/ICSI (2020)