Major Proglucagon Fragment (MPGF) ELISA

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The Major Proglucagon Fragment (MPGF) enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of MPGF in EDTA plasma and other biological fluids. This kit is intended for Research Use Only and is not for use in diagnostic or therapeutic procedures.

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Packaging

96 well microtiter

Detection

HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter

Dynamic Range

6, 0.05-3 ng/mL

Limit of Detection

0.003 ng/mL

Sample Size

25 µL

Sample Type

Plasma

Assay Time

2.5 hours

Species Reactivity

Human

Shelf Life

24 months

Availability

Worldwide

Major Proglucagon Fragment (MPGF) is an 86-amino acid hormone secreted from the pancreas1-3. MPGF is identified as the Carboxy terminal portion of proglucagon that contains two glucagon-related sequences. The MPGF sequence is highly conserved among mammals. Tissue specific processing of proglucagon in the pancreas releases MPGF. Intestinal processing of MPGF releases the Glucagon-Like Peptides 1 and 2 (GLP-1 and GLP-2)1-5. Measuring the circulating levels of MPGF will help in understanding the defective or abnormal metabolic pathways leading to diabetes and obesity. This kit is designed to measure MPGF.

References:
1. Müller et al., (2017) The New Biology and Pharmacology of Glucagon. Physiol Rev 97: 721–766.
2. Drucker DJ, Habener JF, Holst JJ Discovery, characterization, and clinical development of the glucagon-like peptides (2017) J Clin Invest. 127(12):4217-4227.
3. Bonic A, Mackin RB (2003) Expression, purification, and PC1-mediated processing of human proglucagon, glicentin, and major proglucagon fragment. Protein Expr Purif 28:15–24.
4. Amato A, Baldassano and Flavia Mulè. GLP2: an underestimated signal for improving glycaemic control and insulin sensitivity. Journal of Endocrinology (2016) 229, R57–R66
5. Baldassano S, Amato A, Rappa F, Cappello F & Mulè F (2016b) Influence of endogenous glucagon like peptide-2 on lipid disorders in mice fed a high fat diet. Endocrine Research.
Vol. 41, Iss. 4.
6. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
7. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available: http://www.cdc.gov/niosh.
8. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.

Technical Documents