The Ultra-Sensitive Inhibin B enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of Inhibin B in human serum and other biological fluids.
Regulatory Status | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
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Packaging | 96 well microtiter |
Detection | HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter |
Dynamic Range | 6, 3.9-542 pg/mL |
Limit of Detection | 0.77 pg/mL |
Sample Size | 50 µL |
Sample Type | Serum |
Assay Time | 1.5 hours |
Species Reactivity | Human |
Shelf Life | 24 months |
Availability | United States |
Inhibin B is a dimeric hormone that is composed of alpha (α) and beta B (βB) subunits. The free alpha subunits usually do not have any physiological effect. Therefore, the bioactivity of the inhibins depends on the formation of a dimeric α-β structure, and only dimeric forms of inhibins are biologically active. Inhibins are protein hormones secreted by granulosa cells of the ovary in the female and sertoli cells of the testis in the male. They selectively suppress the secretion of pituitary follicle stimulating hormone (FSH) and also have local paracrine actions in the gonads. Inhibin B levels have been reported in sertoli cell function (potential marker for spermatogenesis and testicular function), ovarian reserve and granulosa cell tumors.
References:
1. HHS Publication, 5th ed., 2007. Biosafety in Microbiological and Biomedical Laboratories. Available http://www.cdc.gov/biosafety/publications/bmbl5/BMBL5
2. DHHS (NIOSH) Publication No. 78–127, August 1976. Current Intelligence Bulletin 13 – Explosive Azide Hazard. Available http:// www.cdc.gov/niosh.
3. Approved Guideline – Procedures for the Handling and Processing of Blood Specimens, H18-A3. 2004. Clinical and Laboratory Standards Institute.
4. Kricka L. Interferences in immunoassays – still a threat. Clin Chem 2000; 46: 1037–1038.
Inhibin B ELISA AL-107
Chinya A, Ratan SK, Aggarwal SK, Garg A, Mishra TK. Association of levels of serum inhibin b and follicle stimulating hormone with testicular vascularity, volume, and echotexture in children with undescended testes.J Indian Assoc Pediatr Surg. 2017 Jan-Mar; 22(1): 3–8. PMC521713
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Istanbullu S, Lahlou N, Chevenne D, Léger J, Carel J, Martinerie L. Constitutional delay of puberty or hypogonadotropic hypogonadism: Diagnostic value of inhibin B and AMH measurements. Abstract presented at 55th European Society for Paediactric Endocrinology; 2016; Sep 10-12; Paris, France.
All Products Cited Inhibin B ELISA AL-107
Jain M, Kalsi AK, Srivastava A, Gupta YK, Halder A. High Serum Estradiol and Heavy Metals Responsible for Human Spermiation Defect-A Pilot Study. J Clin Diagn Res. 2016 Dec;10(12):RC09-RC13. PMID 28208955
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Kalra B, Patel A, Kommareddy V, Ganapathy K, Mistry S, Budhavarapu V, Savjani G, Pasternack A, Ritvos O, Hyvonen M, Kumar A. Development of Novel Specific ELISAs for all Inhibins, Activins and their Binding Proteins FST and FSTL3. Poster presented at 98th Annual Endocrine Society Meeting; 2016 Apr 1-3; Boston, MA.
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Kim A, Ebbert K, Cordeiro MH, Romero M, Zhu J, Serna VA, Whelan KA, Woodruff TK, Kurita T. Cell Autonomous Phosphoinositide 3-Kinase Activation in Oocytes Disrupts Normal Ovarian Function Through Promoting Survival and Overgrowth of Ovarian Follicles. Endocrinology. 2015 Apr; 156(4): 1464–1476. PMID 25594701
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Kim SY, Ebbert K, Cordeiro MH, Romero MM, Whelan KA, Suarez AA, Woodruff TK, Kurita T. Constitutive Activation of PI3K in Oocyte Induces Ovarian Granulosa Cell Tumors. Cancer Res. 2016 Jul 1;76(13):3851-61. PMID 27197196
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Kumar A, Kalra B, Patel A, Shah S. Development of a Sensitive Inhibin B ELISA Optimized to Eliminate False Positive Results. Poster session presented at: American Association for Clinical Chemistry; 2013; Jul 30-Aug 1; Houston, TX.
All Products Cited Inhibin B ELISA AL-107
Lahlou N, Santullli P, Guibourdenche J, Beldjord C, Kottler, M, Chapron C, Gompel A. Unexpected Inhibin B, Insulin-like Peptide 3, and Testosterone Production by Mother Ovaries in a Pregnancy with Sustained High hCG Levels. A Clue to Hyperreactio Luteinalis? Poster session presented at: Endocrine Society’s 96th Annual Meeting; 2014; June 21-24; Chicago, IL.
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Liu DM, Torchen LC, Sung Y, Paparodis R, Legro RS, Grebe SK, Singh RJ, Taylor RL, Dunaif A Evidence for gonadotrophin secretory and steroidogenic abnormalities in brothers of women with polycystic ovary syndrome. Human Reproduction, Vol.29, No.12 pp. 2764–2772, 2014 Oct 21. PMID 25336708
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Perakakis N, Upadhyay J, Ghaly W, Chen J, Chrysafi P, Anastasilakis AD, Mantzoros CS. Regulation of the activins‐follistatins‐inhibins axis by energy status: Impact on reproductive function. Metabolism. 2018 May 9. pii: S0026‐0495(18)30120‐3.
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Perakakis N, Upadhyay J, Ghaly W, Chen J, Mantzoros CS. Leptin‐dependent and leptin‐independent pathways linking energy sufficiency with reproduction. Poster session presented at the Obesity Week Meeting; 2017 Oct 29‐Nov 2; Washington DC.
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Manente L, Pecoraro S, Picillo E, Gargiulo U, Gargiulo P, De Luca A, Politano L. Molecular evidence of apoptotic pathway activation in semen samples with high DNA fragmentation. In Vivo. 2015 Mar-Apr;29(2):289-94. PMID 25792659
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Ting A, Savjani G, Kalra B, Kumar A, Stouffer R, Zelinski M. Dynamics of Growth and Differentiation Factor‐9 and Inhibin B Production in Macaque Developing Follicles During 3‐Dimensional Culture. Poster Presentation at ASRM 2017 Oct 28‐Nov 1, San Antonio TX.
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Torchen L, Kumar A, Kalra B, Savjani G, Sisk R, Legro R, Dunaif A. Brothers and Fathers of Women with PCOS Have Altered Testicular Function. Poster session presented at: Endocrine Society’s 96th Annual Meeting; 2014; June 21-24; Chicago, IL.
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Torchen LC, Kumar A, Kalra B, Savjani G, Sisk R, Legro RS, Dunaif A. Increased antim€ullerian hormone levels and other reproductive endocrine changes in adult male relatives of women with polycystic ovary syndrome. Fertil Steril. 2016 Jul;106(1):50-55. PMID 27042970
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Xiao S, Coppeta JR, Rogers HB, Isenberg BC, Zhu J, Olalekan SA, McKinnon KE, Dokic D, Rashedi AS, Haisenleder DJ, Malpani SS, Arnold-Murray CA, Chen K, Jiang M, Bai L, Nguyen CT, Zhang J, Laronda MM, Hope TJ, Maniar KP, Pavone ME, Avram MJ, Sefton EC, Getsios S, Burdette JE, Kim JJ, Borenstein JT, Woodruff TK. A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle. Nat Commun. 2017 Mar 28;8:14584. PMID 28350383
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Xingming Z, Qian Y. Relationship between inhibin B and different clinical phenotypes of polycystic ovary syndrome. Chinese Journal of Practical Gynecology and Obstetrics. 2017 eleven pages 1183‐1188.
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Zhou W, Fang F, Zhu W, Chen ZJ, Du Y, Zhang J. Bisphenol A and Ovarian Reserve among Infertile Women with Polycystic Ovarian Syndrome. Int J Environ Res Public Health. 2016 Dec 27;14(1). Pii: E18. PMID 28036005
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