GLP-1 ELISA

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The Glucagon-like peptide-1 (GLP-1) enzyme linked immunosorbent assay (ELISA) kit provides materials for the quantitative measurement of GLP-1 in EDTA plasma and other biological fluids.

Regulatory Status

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Packaging

96 well microtiter

Detection

HRP-based ELISA, colorimetric detection by dual wavelength absorbance at 450 nm and 630 nm as reference filter

Dynamic Range

6, 15-213 pg/mL

Limit of Detection

1.9 pg/mL

Sample Size

50 µL

Sample Type

Plasma

Assay Time

2.5 hours

Species Reactivity

Human, Mouse, Rat

Shelf Life

24 months

Availability

Worldwide

Glucagon-like peptide-1 (GLP-1) is a 30-amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after selective cleavage by dipeptidyl peptidase-4 (DPP-4). GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity.1-5 This kit is designed to measures total of GLP-1.

References:

  1. Gareth E. Lim and Patricia L. Brubaker., Glucagon-Like Peptide 1 Secretion by the L-Cell. Diabetes 2006 Dec; 55(Supplement 2): S70-S77.
  2. Holst, Jens J. et al., Glucagon-like peptide-1, glucose homeostasis and diabetes. Trends in Molecular Medicine 2008; 14(4): 161 – 168.
  3. Bak MJ, et.al., Specificity and sensitivity of commercially available assays for glucagon-like peptide-1 (GLP-1): implications for GLP-1 measurements in clinical studies. Diabetes Obes Metab. 2014 Nov;16(11):1155-64.
  4. Caporarello N,et.al., Insulin receptor signaling and glucagon-like peptide 1 effects on pancreatic beta cells. PLoS One. 2017 Aug 2;12(8): 1-11.
  5. Lee YS, Jun HS. Anti-diabetic actions of glucagon-like peptide-1 on pancreatic beta-cells. Metabolism. 2014 Jan;63(1):9-19.Sandoval DA and D’Alessio DA. Physiology of Proglucagon Peptides: Role of Glucagon and GLP-1 in Health and Disease. Physiol Rev 95:513-548.

GLP‐1 ELISA AL‐172

 

Kumar A, Kalra B, Mistry S, Patel AS, Budhavarapu V, Chatterji T, Savjani G, Krishna R, D’Alessio D. Development of Novel Specific and Sensitive ELISAs for Proglucagon‐Derived Peptides. Poster presented at 69th AACC Meeting; 2017 July 30‐Aug 3; San Diego, CA

All Products Cited: Glucagon ELISA AL‐157, GLP‐1 ELISA AL‐172, Oxyntomodulin ELISA AL‐139

Technical Literature